19 March 2015 Mapping of the Samara city by definition of areas with hydrogen degassing using Raman spectroscopy
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One of the complicating factors for environmental situation is degassing of land. The high concentrations of hydrogen near the bearing metal structures can weaken them as a result of embrittlement. Therefore, the study problems of hydrogen concentration in the soil and hydrogen influence on living organisms are relevant. However, the exit of deep hydrogen has a volley character. This problem can be solved by the plant bioobjects as the local integral indicators. The dandelion (Taráxacum) was selected as the research object. The collection of objects was produced from the degassing zone and a zone without degassing. Selection of degassing zone was driven by information that was provided by the Volga branch of the Institute of Geology and Exploration of fossil fuels of the Samara Region. Experimental studies of the hydrogen influence on the optical properties of plants were conducted using a complex of Raman spectroscopy and confocal microscopy. Laboratory and field research were conducted. Raman spectroscopy was implemented using the experimental stand that includes a radiation source, a fiber system for collect and feed of radiation and SR-303i spectrophotometer with integrated digital camera ANDOR DV-420A-OE (1024 * 256). The experimental stand allows to work in the spectral range of 180 - 1200 nm and with a registration accuracy about 0.2 nm. A detailed analysis of the structural changes in plant cells under the hydrogen influence was performed by confocal microscopy.
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E. V. Timchenko, E. V. Timchenko, P. E. Timchenko, P. E. Timchenko, L. A. Zherdeva Taskina, L. A. Zherdeva Taskina, N. V. Тregub, N. V. Тregub, E. A. Selezneva, E. A. Selezneva, "Mapping of the Samara city by definition of areas with hydrogen degassing using Raman spectroscopy", Proc. SPIE 9448, Saratov Fall Meeting 2014: Optical Technologies in Biophysics and Medicine XVI; Laser Physics and Photonics XVI; and Computational Biophysics, 94480K (19 March 2015); doi: 10.1117/12.2179510; https://doi.org/10.1117/12.2179510

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