Antimicrobial blue light therapy for Candida albicans burn infection in mice

Yunsong Zhang; Yucheng Wang; Clinton K. Murray; Michael R. Hamblin; Ying Gu; Tianhong Dai

doi:10.1117/12.2178232

22 May 2015 Antimicrobial blue light therapy for Candida albicans burn infection in mice

Yunsong Zhang, Yucheng Wang, Clinton K. Murray, Michael R. Hamblin, Ying Gu, Tianhong Dai

Proceedings Volume 9467, Micro- and Nanotechnology Sensors, Systems, and Applications VII; 94670K (2015) https://doi.org/10.1117/12.2178232
Event: SPIE Defense + Security, 2015, Baltimore, MD, United States

Abstract

In this preclinical study, we investigated the utility of antimicrobial blue light therapy for Candida albicans infection in acutely burned mice. A bioluminescent strain of C. albicans was used. The susceptibilities to blue light inactivation were compared between C. albicans and human keratinocyte. In vitro serial passaging of C. albicans on blue light exposure was performed to evaluate the potential development of resistance to blue light inactivation. A mouse model of acute thermal burn injury infected with the bioluminescent strain of C. albicans was developed. Blue light (415 nm) was delivered to mouse burns for decolonization of C. albicans. Bioluminescence imaging was used to monitor in real time the extent of fungal infection in mouse burns. Experimental results showed that C. albicans was approximately 42-fold more susceptible to blue light inactivation in vitro than human keratinocyte (P=0.0022). Serial passaging of C. albicans on blue light exposure implied a tendency for the fungal susceptibility to blue light inactivation to decrease with the numbers of passages. Blue light reduced fungal burden by over 4-log₁₀ (99.99%) in acute mouse burns infected with C. albicans in comparison to infected mouse burns without blue light therapy (P=0.015).

Citation Download Citation

Yunsong Zhang, Yucheng Wang, Clinton K. Murray, Michael R. Hamblin, Ying Gu, and Tianhong Dai "Antimicrobial blue light therapy for Candida albicans burn infection in mice", Proc. SPIE 9467, Micro- and Nanotechnology Sensors, Systems, and Applications VII, 94670K (22 May 2015); https://doi.org/10.1117/12.2178232

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