A plastic optical fiber biosensor for e. coli

Domingos M. C. Rodrigues; Regina C. S. B. Allil; Vanessa M. Queiroz; Rafaela N. Lopes; Alexandre Silva Allil; Marcelo M. Werneck

doi:10.1117/12.2177246

13 May 2015 A plastic optical fiber biosensor for e. coli

Domingos M. C. Rodrigues, Regina C. S. B. Allil, Vanessa M. Queiroz, Rafaela N. Lopes, Alexandre Silva Allil, Marcelo M. Werneck

Author Affiliations +

Proceedings Volume 9486, Advanced Environmental, Chemical, and Biological Sensing Technologies XII; 94860T (2015) https://doi.org/10.1117/12.2177246
Event: SPIE Sensing Technology + Applications, 2015, Baltimore, MD, United States

Abstract

This work presents a novel, fast response time, plastic optic fiber (POF) biosensor to detect Escherichia coli. Different forms of probes in U-shaped format were tested: U-shaped with different radii, coil-shaped and meander-shaped. In the calibration process we used solutions of sucrose for obtaining refractive indexes (RI) in the range 1.33 – 1.39 RIUequivalent of water and bacteria, respectively. The POF probes were functionalized with antibody anti-Escherichia coli serotype O55 and tested with bacteria concentrations of 10⁴, 10⁶ and 10⁸ colonies forming unities/mL (CFU/mL). The optoelectronic setup consists of an 880 nm LED connected to the U-shaped probe driven by a current source controlled by an Arduino Microcontroller. At the opposite fiber end, the light received by a photodetector is amplified and read by the A/D port of the microcontroller. The output voltage decreases as the external RI increases, accordingly to the bacteria that is captured by the antibody fixed in the biosensor. The paper presents results obtained with the different shapes of probes tested with the same setup and biochemical protocol. In conclusion the system shows good capabilities of having concentration measurements results in less than 10 minutes with uncertainty of 10^-4 RIU enabling a future capacity to read concentration of 10³ CFU/mL.

Citation Download Citation

Domingos M. C. Rodrigues, Regina C. S. B. Allil, Vanessa M. Queiroz, Rafaela N. Lopes, Alexandre Silva Allil, and Marcelo M. Werneck "A plastic optical fiber biosensor for e. coli", Proc. SPIE 9486, Advanced Environmental, Chemical, and Biological Sensing Technologies XII, 94860T (13 May 2015); https://doi.org/10.1117/12.2177246

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