Remote characterization of biological specimens using all-optical frequency-domain photoacoustic microscopy

Ashwin Sampathkumar

doi:10.1117/12.2177426

21 May 2015 Remote characterization of biological specimens using all-optical frequency-domain photoacoustic microscopy

Ashwin Sampathkumar

Author Affiliations +

Proceedings Volume 9487, Smart Biomedical and Physiological Sensor Technology XII; 94870H (2015) https://doi.org/10.1117/12.2177426
Event: SPIE Sensing Technology + Applications, 2015, Baltimore, MD, United States

Abstract

In this paper, we describe a narrow-bandwidth generation and detection of photoacoustic (PA) signals in biological specimens using frequency-domain photoacoustics. An intensity-modulated laser was used for PA generation, and a homodyne Michelson interferometer coupled to a lock-in amplifier was used for optical PA detection. The amplitude and phase of the PA signal were measured at the modulation frequency as the frequency was swept over the bandwidth of interest. A synthesized pulse response was obtained using time-domain reconstruction and the absorber map was estimated using k-space reconstruction methods. Experimental results obtained from 500-μm graphite rods embedded in tissue-mimicking phantoms and slide-mounted tissue samples are presented along with their respective time-domain and time-reversal reconstruction maps.

Citation Download Citation

Ashwin Sampathkumar "Remote characterization of biological specimens using all-optical frequency-domain photoacoustic microscopy", Proc. SPIE 9487, Smart Biomedical and Physiological Sensor Technology XII, 94870H (21 May 2015); https://doi.org/10.1117/12.2177426

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

;

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Tissue optics

Modulation

Tissues

Optical amplifiers

Transducers

Geometrical optics

Signal detection

Show All Keywords

Keywords/Phrases

Search In:

Publication Years