13 May 2015 Lab-on-a-chip enabled HLA diagnostic: combined sample preparation and real time PCR for HLA-B57 diagnosis
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Abstract
The diverse human HLA (human leukocyte antigen) system is responsible for antigen presentation and recognition. It is essential for the immune system to maintain a stable defense line, but also is also involved in autoimmunity as well as metabolic disease. HLA-haplotype (HLA-B27), for instance, is associated with inflammatory diseases such as Bechterew's disease. The administration of the HIV drug Abacavir in combination with another HLA-haplotype (HLAB57) is associated with severe hypersensitivity reactions. Accordingly, the HLA status has to be monitored for diagnosis or prior to start of therapy. Along this line, a miniaturized microfluidic platform has been developed allowing performing the complete analytical process from “sample-in” to “answer-out” in a point-of-care environment. The main steps of the analytical cascade inside the integrated system are blood cell lysis and DNA isolation, DNA purification, real-time PCR and quantitative monitoring of the rise of a fluorescent signal appearing during the PCR based sequence amplification. All bio-analytical steps were intended to be performed inside one chip and will be actuated, controlled and monitored by a matching device. This report will show that all required processes are established and tested and all device components work well and interact with the functional modules on the chips in a harmonized fashion.
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Claudia Gärtner, Claudia Gärtner, Holger Becker, Holger Becker, Nadine Hlawatsch, Nadine Hlawatsch, Richard Klemm, Richard Klemm, Christian Moche, Christian Moche, Sebastian Schattschneider, Sebastian Schattschneider, Rainer Frank, Rainer Frank, Andreas Willems, Andreas Willems, } "Lab-on-a-chip enabled HLA diagnostic: combined sample preparation and real time PCR for HLA-B57 diagnosis", Proc. SPIE 9490, Advances in Global Health through Sensing Technologies 2015, 94900F (13 May 2015); doi: 10.1117/12.2178897; https://doi.org/10.1117/12.2178897
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