21 May 2015 Rapid detection of EBOLA VP40 in microchip immunofiltration assay
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Abstract
In the spring of 2014, the Ebola virus (EBOV) strain Zaire caused a dramatic outbreak in several regions of West Africa. The RT-PCR and antigen capture diagnostic proved to be effective for detecting EBOV in blood and serum. In this paper, we present data of a rapid antigen capture test for the detection of VP40. The test was performed in a microfluidic chip for immunofiltration analysis. The chip integrates all necessary assay components. The analytical sensitivity of the rapid test was 8 ng/ml for recombinant VP40. In serum and whole blood samples spiked with virus culture material, the detection limit was 2.2 x 102 PFU/ml. The performance data of the rapid test (15 min) are comparable to that of the VP40 laboratory ELISA.
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Peter Miethe, Peter Miethe, Dominik Gary, Dominik Gary, Nadine Hlawatsch, Nadine Hlawatsch, Anne-Marie Gad, Anne-Marie Gad, } "Rapid detection of EBOLA VP40 in microchip immunofiltration assay", Proc. SPIE 9490, Advances in Global Health through Sensing Technologies 2015, 94900H (21 May 2015); doi: 10.1117/12.2184649; https://doi.org/10.1117/12.2184649
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