Selective multiphoton excitation by parametrically shaped laser pulses

A. Lindinger

doi:10.1117/12.2087110

18 May 2015 Selective multiphoton excitation by parametrically shaped laser pulses

A. Lindinger

Proceedings Volume 9503, Nonlinear Optics and Applications IX; 95030K (2015) https://doi.org/10.1117/12.2087110
Event: SPIE Optics + Optoelectronics, 2015, Prague, Czech Republic

Abstract

Laser pulse shaping is reported for applications on multiphoton processes in dye molecules. Particularly phase-tailored pulse shapes are employed for two-photon excited fluorescence of dyes in a liquid environment, also at the distal end of an optical fiber, in order to improve the contrast between dye markers having similar excitation spectra. Precompensation of the optical fiber properties is utilized by analytical pulse shaping in order to receive specific parametric pulse forms after the fiber. This will lead to new endoscopic imaging applications with an increased fluorescence contrast. Moreover, selective excitation is also demonstrated for three-photon transitions of the two dyes, p-Terphenyl (PTP) and BM-Terphenyl (BMT), in solution by using shaped pulses without a fiber. A good agreement between experiment and theoretical simulation is obtained. With this approach it is possible to achieve a considerable change of the fluorescence contrast between the two dyes which is relevant for imaging applications of biological molecules.

Citation Download Citation

A. Lindinger "Selective multiphoton excitation by parametrically shaped laser pulses", Proc. SPIE 9503, Nonlinear Optics and Applications IX, 95030K (18 May 2015); https://doi.org/10.1117/12.2087110

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available