5 May 2015 A silicon-based peptide biosensor for label-free detection of cancer cells
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Sensitive and accurate detection of cancer cells plays a crucial role in diagnosis of cancer and minimal residual disease, so being one of the most hopeful approaches to reduce cancer death rates. In this paper, a strategy for highly selective and sensitive detection of lymphoma cells on planar silicon-based biosensor has been evaluated. In this setting an Idiotype peptide, able to specifically bind the B-cell receptor (BCR) of A20 cells in mice engrafted with A20 lymphoma, has been covalently linked to the sensor active surface and used as molecular probe. The biochip here presented showed a coverage efficiency of 85% with a detection efficiency of 8.5×10-3 cells/μm2. The results obtained suggested an efficient way for specific label-free cell detection by using a silicon-based peptide biosensor. In addition, the present recognition strategy, besides being useful for the development of sensing devices capable of monitoring minimal residual disease, could be used to find and characterize new specific receptor-ligand interactions through the screening of a recombinant phage library.
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Nicola M. Martucci, Nicola M. Martucci, Ilaria Rea, Ilaria Rea, Immacolata Ruggiero, Immacolata Ruggiero, Monica Terracciano, Monica Terracciano, Luca De Stefano, Luca De Stefano, Nunzia Migliaccio, Nunzia Migliaccio, Principia Dardano, Principia Dardano, Paolo Arcari, Paolo Arcari, Ivo Rendina, Ivo Rendina, Annalisa Lamberti, Annalisa Lamberti, "A silicon-based peptide biosensor for label-free detection of cancer cells", Proc. SPIE 9506, Optical Sensors 2015, 95061T (5 May 2015); doi: 10.1117/12.2178137; https://doi.org/10.1117/12.2178137

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