1 June 2015 Magnetic microfluidic system for isolation of single cells
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Abstract
This paper presents the design and realization of a compact, portable and cost effective microfluidic system for isolation and detection of rare circulating tumor cells (CTCs) in suspension. The innovative aspect of the proposed isolation method is that it utilizes superparamagnetic particles (SMPs) to label CTCs and then isolate those using microtraps with integrated current carrying microconductors. The magnetically labeled and trapped CTCs can then be detected by integrated magnetic microsensors e.g. giant magnetoresistive (GMR) or giant magnetoimpedance (GMI) sensors. The channel and trap dimensions are optimized to protect the cells from shear stress and achieve high trapping efficiency. These intact single CTCs can then be used for additional analysis, testing and patient specific drug screening. Being able to analyze the CTCs metastasis-driving capabilities on the single cell level is considered of great importance for developing patient specific therapies. Experiments showed that it is possible to capture single labeled cells in multiple microtraps and hold them there without permanent electric current and magnetic field.
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Richard Mitterboeck, Richard Mitterboeck, Georgios Kokkinis, Georgios Kokkinis, Theocharis Berris, Theocharis Berris, Franz Keplinger, Franz Keplinger, Ioanna Giouroudi, Ioanna Giouroudi, } "Magnetic microfluidic system for isolation of single cells", Proc. SPIE 9518, Bio-MEMS and Medical Microdevices II, 951809 (1 June 2015); doi: 10.1117/12.2181194; https://doi.org/10.1117/12.2181194
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