1 June 2015 Optical detection of two-color-fluorophore barcode for nanopore DNA sensing
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A simple schematic on parallel optical detection of two-fluorophore barcode for single-molecule nanopore sensing is presented. The chosen two fluorophores, ATTO-532 and DY-521-XL, emitting in well-separated spectrum range can be excited at the same wavelength. A beam splitter was employed to separate signals from the two fluorophores and guide them to the same CCD camera. Based on a conventional microscope, sources of background in the nanopore sensing system, including membranes, compounds in buffer solution, and a detection cell was characterized. By photoluminescence excitation measurements, it turned out that silicon membrane has a negligible photoluminescence under the examined excitation from 440 nm to 560 nm, in comparison with a silicon nitrite membrane. Further, background signals from the detection cell were suppressed. Brownian motion of 450 bps DNA labelled with single ATTO-532 or DY-521-XL was successfully recorded by our optical system.
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M. Zhang, M. Zhang, I. Sychugov, I. Sychugov, T. Schmidt, T. Schmidt, J. Linnros, J. Linnros, } "Optical detection of two-color-fluorophore barcode for nanopore DNA sensing", Proc. SPIE 9519, Nanotechnology VII, 95190G (1 June 2015); doi: 10.1117/12.2179626; https://doi.org/10.1117/12.2179626

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