Observation of Brownian motion of a micrometer-size bead in water by off-axis digital holographic microscopy

Yoon-Sung Bae; Phil-Jun Jeon; Hee-Jung Lee

doi:10.1117/12.2189433

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8 July 2015 Observation of Brownian motion of a micrometer-size bead in water by off-axis digital holographic microscopy

Yoon-Sung Bae, Phil-Jun Jeon, Hee-Jung Lee

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Proceedings Volume 9523, International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015; 95230A (2015) https://doi.org/10.1117/12.2189433
Event: International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015, 2015, Jeju, Korea, Republic of

Abstract

An effective volumetric measurement method for determination of the 3D position of a single particle based on off-axis digital holographic microscopy is presented in this paper. 3 μm polystyrene bead suspended water is used as a particle to be traced in our experiment. We have demonstrated the feasibility of our method by observing the bead undergoing Brownian motion in water by implemented setup. For fast determination, a series of transverse intensity images are numerically reconstructed from a single hologram of the bead, then centroid technique is applied to the reconstructed images for enhancing the position resolution. Nano-meter scale resolution with a frame rate of 30 is achieved in this experiment in both lateral and transverse direction.

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Yoon-Sung Bae, Phil-Jun Jeon, and Hee-Jung Lee "Observation of Brownian motion of a micrometer-size bead in water by off-axis digital holographic microscopy", Proc. SPIE 9523, International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015, 95230A (8 July 2015); https://doi.org/10.1117/12.2189433

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