22 June 2015 Parameter optimization of phase microscope with the interferometer as a spatial phase modulator
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Spatial light interference microscopy (SLIM) is the optical method for visualization cell structure and measurement its dynamics. The distinctive feature of SLIM technique is introducing the additional spatial phase modulation of optical field in system of phase contrast microscope. In the currently known optical schemes of phase microscope the additional phase modulation is created by the liquid crystal spatial phase modulator (LCPM). It produces the phase shifts to the π/2, introducing the phase contrast between undiffracted and diffracted light from the sample. In our work we use the Michelson interferometer type optical system for introducing the phase delay between two waves. The phase shift is produced by micro displacement of one of the mirrors of the interferometer with the help of the piezoelectric element. Such modification allows setting the path difference of fraction of the wavelength between the undiffracted and diffracted components of the optical field, so it’s possible to get quantitative phase image of the object. This modernization of standard scheme of SLIM improves its performance due to higher speed of piezoelectric element comparing with speed of LCPM. The quality of result images of the investigated object depend on technical parameters of the optical scheme of microscope, including the size of illumination system aperture diaphragm. The purpose of this work is to investigate the influence of the aperture diaphragm on the phase image quality of RBC, which will be used further for the measurement RBC parameters and its dynamics.
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O. A. Izotova, O. A. Izotova, V. P. Ryabukho, V. P. Ryabukho, } "Parameter optimization of phase microscope with the interferometer as a spatial phase modulator", Proc. SPIE 9529, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials II, 95291H (22 June 2015); doi: 10.1117/12.2184678; https://doi.org/10.1117/12.2184678

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