19 June 2015 Tumor tissue characterization using polarization-sensitive second harmonic generation microscopy
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Proceedings Volume 9531, Biophotonics South America; 95310C (2015) https://doi.org/10.1117/12.2180969
Event: SPIE Biophotonics South America, 2015, Rio de Janeiro, Brazil
Changes in the ultrastructure of collagen in various tumor and non-tumor human tissues including lung, pancreas and thyroid were investigated ex vivo by a polarization-sensitive second harmonic generation (SHG) microscopy technique referred to as polarization-in, polarization-out (PIPO) SHG. This involves measuring the orientation of the linear polarization of outgoing SHG as a function of the linear polarization orientation of incident laser radiation. From the PIPO SHG data, the second-order nonlinear optical susceptibility tensor component ratio, χ(2) ZZZ’/χ(2) ZXX’, for each pixel of the SHG image was obtained and presented as color-coded maps. Further, the orientation of collagen fibers in the tissue was deduced. Since the χ(2) ZZZ’/χ(2) ZXX’ values represent the organization of collagen in the tissue, theses maps revealed areas of altered collagen structure (not simply concentration) within tissue sections. Statistically-significant differences in χ(2) ZZZ’/χ(2) ZXX’ were found between tumor and non-tumor tissues, which varied from organ to organ. Hence, PIPO SHG microscopy could potentially be used to aid pathologists in diagnosing cancer. Additionally, PIPO SHG microscopy could aid in characterizing the structure of collagen in other collagen-related biological processes such as wound repair.
© (2015) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Danielle Tokarz, Danielle Tokarz, Richard Cisek, Richard Cisek, Ahmad Golaraei, Ahmad Golaraei, Serguei Krouglov, Serguei Krouglov, Roya Navab, Roya Navab, Carolyn Niu, Carolyn Niu, Shingo Sakashita, Shingo Sakashita, Kazuhiro Yasufuku, Kazuhiro Yasufuku, Ming-Sound Tsao, Ming-Sound Tsao, Sylvia L. Asa, Sylvia L. Asa, Virginijus Barzda, Virginijus Barzda, Brian C. Wilson, Brian C. Wilson, } "Tumor tissue characterization using polarization-sensitive second harmonic generation microscopy", Proc. SPIE 9531, Biophotonics South America, 95310C (19 June 2015); doi: 10.1117/12.2180969; https://doi.org/10.1117/12.2180969

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