19 June 2015 Fluorescence spectroscopy for assessment of liver transplantation grafts concerning graft viability and patient survival
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Proceedings Volume 9531, Biophotonics South America; 95310X (2015) https://doi.org/10.1117/12.2180730
Event: SPIE Biophotonics South America, 2015, Rio de Janeiro, Brazil
Abstract
Evaluating transplantation grafts at harvest is essential for its success. Laser-induced fluorescence spectroscopy (LIFS) can help monitoring changes in metabolic/structural conditions of tissue during transplantation. The aim of the present study is to correlate LIFSobtained spectra of human hepatic grafts during liver transplantation with post-operative patients' mortality rate and biochemical parameters, establishing a method to exclude nonviable grafts before implantation. Orthotopic liver transplantation, piggyback technique was performed in 15 patients. LIFS was performed under 408nm excitation. Collection was performed immediately after opening donor’s abdominal cavity, after cold perfusion, end of back-table period, and 5 min and 1 h after warm perfusion at recipient. Fluorescence information was compared to lactate, creatinine, bilirubin and INR levels and to survival status. LIFS was sensitive to liver changes during transplantation stages. Study-in-progress; initial results indicate correlation between fluorescence and life/death status of patients.
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José D. Vollet Filho, José D. Vollet Filho, Marina R. da Silveira, Marina R. da Silveira, Orlando Castro-e-Silva, Orlando Castro-e-Silva, Vanderlei S. Bagnato, Vanderlei S. Bagnato, Cristina Kurachi, Cristina Kurachi, } "Fluorescence spectroscopy for assessment of liver transplantation grafts concerning graft viability and patient survival", Proc. SPIE 9531, Biophotonics South America, 95310X (19 June 2015); doi: 10.1117/12.2180730; https://doi.org/10.1117/12.2180730
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