19 June 2015 Micro energy-dispersive x-ray fluorescence spectrometry study of dentin coating with nanobiomaterials
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Proceedings Volume 9531, Biophotonics South America; 95313B (2015) https://doi.org/10.1117/12.2180965
Event: SPIE Biophotonics South America, 2015, Rio de Janeiro, Brazil
Abstract
New biomaterials such as multi-walled carbon nanotubes oxide/graphene oxide (MWCNTO/GO), nanohydroxyapatite (nHAp) and combination of them together or not to acidulated phosphate fluoride gel (F) have been tested as protective coating before root dentin erosion. Fourteen bovine teeth were cleaned, polished, divided into two parts (n=28) and assigned to seven groups: (Control) – without previous surface treatment; F treatment; nHAp; MWCNTO/GO; F+nHAp; F+MWCNTO/GO and F+MWCNTO/GO/nHAp composites. Each sample had two sites of pre-treatments: acid etched area and an area without treatment. After the biomaterials application, the samples were submitted to six cycles (demineralization: orange juice, 10 min; remineralization: artificial saliva, 1 h). Micro energy-dispersive X-ray fluorescence spectrometry (μ-EDXRF) mapping area analyses were performed after erosive cycling on both sites (n=84). μ-EDXRF mappings showed that artificial saliva and MWCNTO/GO/nHAp/F composite treatments produced lower dentin demineralization than in the other groups. Exposed dentin tubules allowed better interaction of nanobiomaterials than in smear layer covered dentin. Association of fluoride with other biomaterials had a positive influence on acid etched dentin. MWCNTO/GO/nHAp/F composite treatment resulted in levels of demineralization similar to the control group.
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Luís Eduardo Silva Soares, Luís Eduardo Silva Soares, Sídnei Nahorny, Sídnei Nahorny, Fernanda Roberta Marciano, Fernanda Roberta Marciano, Hudson Zanin, Hudson Zanin, Anderson de Oliveira Lobo, Anderson de Oliveira Lobo, } "Micro energy-dispersive x-ray fluorescence spectrometry study of dentin coating with nanobiomaterials", Proc. SPIE 9531, Biophotonics South America, 95313B (19 June 2015); doi: 10.1117/12.2180965; https://doi.org/10.1117/12.2180965
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