19 June 2015 Optical fluorescence spectroscopy to detect hepatic necrosis after normothermic ischemia: animal model
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Proceedings Volume 9531, Biophotonics South America; 95313V (2015) https://doi.org/10.1117/12.2180897
Event: SPIE Biophotonics South America, 2015, Rio de Janeiro, Brazil
Abstract
Liver transplantation is a well-established treatment for liver failure. However, the success of the transplantation procedure depends on liver graft conditions. The tissue function evaluation during the several transplantation stages is relevant, in particular during the organ harvesting, when a decision is made concerning the viability of the graft. Optical fluorescence spectroscopy is a good option because it is a noninvasive and fast technique. A partial normothermic hepatic ischemia was performed in rat livers, with a vascular occlusion of both median and left lateral lobes, allowing circulation only for the right lateral lobe and the caudate lobe. Fluorescence spectra under excitation at 532 nm (doubled frequency Nd:YAG laser) were collected using a portable spectrometer (USB2000, Ocean Optics, USA). The fluorescence emission was collected before vascular occlusion, after ischemia, and 24 hours after reperfusion. A morphometric histology analysis was performed as the gold standard evaluation ─ liver samples were analyzed, and the percentage of necrotic tissue was obtained. The results showed that changes in the fluorescence emission after ischemia can be correlated with the amount of necrosis evaluated by a morphometric analysis, the Pearson correlation coefficient of the generated model was 0.90 and the root mean square error was around 20%. In this context, the laser-induced fluorescence spectroscopy technique after normothermic ischemia showed to be a fast and efficient method to differentiate ischemic injury from viable tissues.
© (2015) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Renan A. Romano, Renan A. Romano, Jose D. Vollet-Filho, Jose D. Vollet-Filho, Sebastião Pratavieira, Sebastião Pratavieira, Jorge L. Fernandez, Jorge L. Fernandez, Cristina Kurachi, Cristina Kurachi, Vanderlei S. Bagnato, Vanderlei S. Bagnato, Orlando Castro-e-Silva, Orlando Castro-e-Silva, Ajith K. Sankarankutty, Ajith K. Sankarankutty, } "Optical fluorescence spectroscopy to detect hepatic necrosis after normothermic ischemia: animal model", Proc. SPIE 9531, Biophotonics South America, 95313V (19 June 2015); doi: 10.1117/12.2180897; https://doi.org/10.1117/12.2180897
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