14 July 2015 Tomographic phase microscopy using optical tweezers
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Abstract
We review our technique for tomographic phase microscopy with optical tweezers [1]. This tomographic phase microscopy approach enables full 3-D refractive-index reconstruction. Tomographic phase microscopy measures quantitatively the 3- D distribution of refractive-index in biological cells. We integrated our external interferometric module with holographic optical tweezers for obtaining quantitative phase maps of biological samples from a wide range of angles. The close-tocommon- path, off-axis interferometric system enables a full-rotation tomographic acquisition of a single cell using holographic optical tweezers for trapping and manipulating with a desired array of traps, while acquiring phase information of a single cell from all different angles and maintaining the native surrounding medium. We experimentally demonstrated two reconstruction algorithms: the filtered back-projection method and the Fourier diffraction method for 3-D refractive index imaging of yeast cells.
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Mor Habaza, Mor Habaza, Barak Gilboa, Barak Gilboa, Yael Roichman, Yael Roichman, Natan T. Shaked, Natan T. Shaked, } "Tomographic phase microscopy using optical tweezers", Proc. SPIE 9536, Advanced Microscopy Techniques IV; and Neurophotonics II, 95360H (14 July 2015); doi: 10.1117/12.2183708; https://doi.org/10.1117/12.2183708
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