15 July 2015 Time-resolved study of microorganisms by Raman spectroscopy
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Abstract
The main goal of our investigations is to focus on the basic physiological mechanisms of microorganisms (yeast and bacteria), exposed to different conditions, by time-resolved Raman spectroscopy. This study provides an insight into the mechanism of targeted stress factors or the influence of different cultivation times on species metabolism in vivo, in realtime and label free. We also focused on time-course study of physico-chemical properties of bacterial cells and cell cytoplasm with respect to the intracellular content of polyhydroxyalkanoates and to the production of yeast lipids or carotenoids.
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Ota Samek, Ota Samek, Andrea Haronikova, Andrea Haronikova, Stanislav Obruca, Stanislav Obruca, Silvie Bernatova, Silvie Bernatova, Jan Jezek, Jan Jezek, Martin Siler, Martin Siler, Katarina Mlynarikova, Katarina Mlynarikova, Pavel Zemanek, Pavel Zemanek, } "Time-resolved study of microorganisms by Raman spectroscopy", Proc. SPIE 9537, Clinical and Biomedical Spectroscopy and Imaging IV, 953703 (15 July 2015); doi: 10.1117/12.2183950; https://doi.org/10.1117/12.2183950
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