A topical fluorescent analogue for virtual hematoxylin and eosin histology in point-of-care ex vivo microscopy

Katherine Elfer; Andrew Sholl; Christopher Miller; J. Quincy Brown

doi:10.1117/12.2183959

15 July 2015 A topical fluorescent analogue for virtual hematoxylin and eosin histology in point-of-care ex vivo microscopy

Katherine Elfer, Andrew Sholl, Christopher Miller, J. Quincy Brown

Author Affiliations +

Proceedings Volume 9537, Clinical and Biomedical Spectroscopy and Imaging IV; 95370K (2015) https://doi.org/10.1117/12.2183959
Event: European Conferences on Biomedical Optics, 2015, Munich, Germany

Abstract

Histological assessment of freshly removed tissue specimens requires accurate and fast analysis in clinical procedures such as diagnostic biopsy and surgical tumor resection. Current histological assessment methods are either time-consuming or damage the tissue beyond the ability to re-analyze post-procedure. We demonstrate a novel dual-stain fluorescent analogue to brightfield Hematoxylin and Eosin for in-procedure histopathology that is both time-efficient and preserves the analyzed tissue for later analysis. H&E-like images are created from the combination of DRAQ5 and Eosin applied to human prostate tissue and animal muscle tissue under confocal microscopy. D&E images are pseduocolored to match H&E coloring, showing near-identical features to brightfield H&E of the same tissue. The histological accuracy, short staining time, and tissue preservation aspects of this dual-stain technique demonstrates its potential to be adopted for use in point-of-care pathology.

Citation Download Citation

Katherine Elfer, Andrew Sholl, Christopher Miller, and J. Quincy Brown "A topical fluorescent analogue for virtual hematoxylin and eosin histology in point-of-care ex vivo microscopy", Proc. SPIE 9537, Clinical and Biomedical Spectroscopy and Imaging IV, 95370K (15 July 2015); https://doi.org/10.1117/12.2183959

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available