15 July 2015 Gold nanorods as photothermal agents and autofluorescence enhancer to track cell death during plasmonic photothermal therapy
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Abstract
The transverse and longitudinal plasmon resonance in gold nanorods can be exploited to localize the photothermal therapy and influence the fluorescence to monitor the treatment outcome at the same time. While the longitudinal plasmon peak contributes to the photothermal effect, the transverse peak can enhance fluorescence. After cells take in PEGylated nanorods through endocytosis, autofluorescence from endogenous fluorophores such as nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) in the mitochondria is enhanced two times, which is a good indicator of the respiratory status of the cell. When cells are illuminated continuously with near infrared laser, the temperature reaches the hyperthermic region within the first four minutes, which demonstrates the efficiency of gold nanorods in photothermal therapy. The cell viability test and autofluorescence intensity show good correlation indicating the progress of cell death over time.
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Ravi Kumar Kannadorai, Ravi Kumar Kannadorai, Geraldine Giap Ying Chiew, Geraldine Giap Ying Chiew, Kathy Qian Luo, Kathy Qian Luo, Quan Liu, Quan Liu, } "Gold nanorods as photothermal agents and autofluorescence enhancer to track cell death during plasmonic photothermal therapy", Proc. SPIE 9537, Clinical and Biomedical Spectroscopy and Imaging IV, 95371B (15 July 2015); doi: 10.1117/12.2183581; https://doi.org/10.1117/12.2183581
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