15 July 2015 Ultra-sensitive detection of biomarker using localized surface plasmon resonance (LSPR) enhanced by ELISA
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We demonstrate a highly sensitive detection of AFP (α-fetoprotein) protein (liver cancer marker) in human serum using the LSPR biosensor. Gold metal nanodot array (MNA) on a glass wafer were fabricated by UV nanoimprint lithography (NIL). After the NIL process using a film stamp and the removal of residual layer via oxygen plasma etching, metal films were deposited using an electron-beam evaporator, followed by the lift-off step. Consequently, the gold MNA was realized on 5-inch glass wafer and the pitch, diameter and height of MNA were 300nm, 150 nm and 20 nm, respectively. We employed observation of LSPR spectra via back-reflection, which provides a stable measurement of LSPR because a probe light does not pass a bio-sample. In addition, one channel among two flow channels was used a control channel, the MNA surface in which was modified with bovine serum albumin, not antibody. After antigen-antibody reaction, the enzyme/precipitation was employed on the MNA (Nano-ELISA). As a result, we could detect AFP in 50L human serum with limit of detection (LOD) of 0.7 zeptomole (10-21 mole).
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Yong-Beom Shin, Yong-Beom Shin, Na rae Jo, Na rae Jo, Ki joong Lee, Ki joong Lee, } "Ultra-sensitive detection of biomarker using localized surface plasmon resonance (LSPR) enhanced by ELISA", Proc. SPIE 9537, Clinical and Biomedical Spectroscopy and Imaging IV, 95371F (15 July 2015); doi: 10.1117/12.2183063; https://doi.org/10.1117/12.2183063

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