15 July 2015 Fluorescence ratiometric classifier for the detection of skin pathologies
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Abstract
Detection of pre-malignant lesions in skin could help in reducing the 5 year patient mortality rates and greatly advancing the quality of life. Current gold standard for the detection of skin pathologies is a tissue biopsy and followed by a series of steps before it is examined under a light microscope by a pathologist. The disadvantage with this method is its invasiveness. Light based biomedical point spectroscopic techniques offers an adjunct technique to invasive tissue pathology. In this context, we have implemented a simple multiplexed ratiometric approach (F470/F560 and F510/F580) based on fluorescence at two excitation wavelengths 378 nm and 445 nm respectively. The emission profile at these excitation wavelengths showed a shift towards the longer wavelengths for melanoma when compared with normal and nevus. At both excitation wavelengths, we observed an increased intensity ratios for normal, followed by nevus and melanoma. This intensity ratios provide a good diagnostic capability in differentiating normal, nevus and melanocytic skin lesions. This method could be applied in vivo because of the simplicity involved in discriminating normal and pathological skin tissues.
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Suresh Anand, Suresh Anand, Riccardo Cicchi, Riccardo Cicchi, Alessandro Cosci, Alessandro Cosci, Susanna Rossari, Susanna Rossari, Dimitrios Kapsokalyvas, Dimitrios Kapsokalyvas, Enrico Baria, Enrico Baria, Vincenza Maio, Vincenza Maio, Daniela Massi, Daniela Massi, Vincenzo De Giorgi, Vincenzo De Giorgi, Nicola Pimpinelli, Nicola Pimpinelli, Francesco Savero Pavone, Francesco Savero Pavone, } "Fluorescence ratiometric classifier for the detection of skin pathologies", Proc. SPIE 9537, Clinical and Biomedical Spectroscopy and Imaging IV, 95371R (15 July 2015); doi: 10.1117/12.2183786; https://doi.org/10.1117/12.2183786
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