Enhancement of upconversion deep-tissue imaging using optical clearing

A. P. Popov; E. V. Khaydukov; A. V. Bykov; V. A. Semchishen; V. V. Tuchin

doi:10.1117/12.2183860

13 July 2015 Enhancement of upconversion deep-tissue imaging using optical clearing

A. P. Popov, E. V. Khaydukov, A. V. Bykov, V. A. Semchishen, V. V. Tuchin

Author Affiliations +

Proceedings Volume 9540, Novel Biophotonics Techniques and Applications III; 95400B (2015) https://doi.org/10.1117/12.2183860
Event: European Conferences on Biomedical Optics, 2015, Munich, Germany

Abstract

We report on the deep-tissue imaging using novel upconversion nanoparticles (UCNPs) β-NaYF₄:Yb³⁺ ,Tm³⁺ (excitation wavelength: 975 nm, detection wavelength: 800 nm) and glycerol as an optical clearing agent to enhance imaging from under 6-mm-thick porcine muscle tissue samples. We show that improvement of luminescent label visualization is caused by transforming of the diffuse label-emitted light into the direct component. This results in the increase in visibility (ratio of the sum and difference of the maximal and minimal intensity) of the label and increase in maximal signal intensity thus making the combination of the phosphors and optical clearing promising for precise detection of tissue-embedded labelled inhomogeneities.

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A. P. Popov, A. P. Popov, E. V. Khaydukov, E. V. Khaydukov, A. V. Bykov, A. V. Bykov, V. A. Semchishen, V. A. Semchishen, V. V. Tuchin, V. V. Tuchin, } "Enhancement of upconversion deep-tissue imaging using optical clearing", Proc. SPIE 9540, Novel Biophotonics Techniques and Applications III, 95400B (13 July 2015); doi: 10.1117/12.2183860; https://doi.org/10.1117/12.2183860

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