Optical measurement of temperature in biological cells under infrared laser light exposure (λ=800 nm)

David Moreau; Claire Lefort; Philippe Leveque; Rod P. O'Connor

doi:10.1117/12.2183805

13 July 2015 Optical measurement of temperature in biological cells under infrared laser light exposure (λ=800 nm)

David Moreau, Claire Lefort, Philippe Leveque, Rod P. O'Connor

Proceedings Volume 9540, Novel Biophotonics Techniques and Applications III; 95400F (2015) https://doi.org/10.1117/12.2183805
Event: European Conferences on Biomedical Optics, 2015, Munich, Germany

Abstract

Interest in the interaction between laser light and biological samples has gained momentum in recent years, particularly in neurobiology, where there is significant potential to stimulate neurons with infrared laser light. Despite recent reports showing the application of infrared light for neurostimulation, the underlying mechanism is still unknown. The two main hypotheses are based on thermal or electrostatic mechanisms. Here, a novel optical method is presented to make temperature measurements in human neural cells under infrared laser excitation (λ=800nm) using the dye Rhodamine B (RhB). The measurement of temperature is based on the property of RhB, a fluorescent dye whose fluorescence intensity decreases linearly with increases in temperature. We present and detail the setup and measurement procedure that has temporal resolution of few milliseconds, based around a fluorescent live-cell imaging microscope used for cellular microfluorimetry experiments.

Citation Download Citation

David Moreau, Claire Lefort, Philippe Leveque, and Rod P. O'Connor "Optical measurement of temperature in biological cells under infrared laser light exposure (λ=800 nm)", Proc. SPIE 9540, Novel Biophotonics Techniques and Applications III, 95400F (13 July 2015); https://doi.org/10.1117/12.2183805

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available