13 July 2015 Full 3D morphology of diatoms flowing in a microfluidic channel by digital holographic microscopy
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Abstract
In this paper, we present a new approach for three-dimensional reconstruction and biovolume estimation of some species of diatoms. An optofluidic platform, composed by an optical tweezer and holographic modulus, is employed to retrieve several holograms at different angular positions, which are processed by the shape from silhouette algorithm to estimate the 3D shape of the cells.
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Roberto Savoia, Roberto Savoia, Pasquale Memmolo, Pasquale Memmolo, Francesco Merola, Francesco Merola, Lisa Miccio, Lisa Miccio, Giuliana D'Ippolito, Giuliana D'Ippolito, Angelo Fontana, Angelo Fontana, Pietro Ferraro, Pietro Ferraro, } "Full 3D morphology of diatoms flowing in a microfluidic channel by digital holographic microscopy", Proc. SPIE 9540, Novel Biophotonics Techniques and Applications III, 95400J (13 July 2015); doi: 10.1117/12.2185016; https://doi.org/10.1117/12.2185016
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