Healing process study in murine skin superficial wounds treated with the blue LED photocoagulator EMOLED

Francesca Rossi; Riccardo Cicchi; Francesca Tatini; Stefano Bacci; Domenico Alfieri; Gaetano De Siena; Francesco S. Pavone; Roberto Pini

doi:10.1117/12.2183670

15 July 2015 Healing process study in murine skin superficial wounds treated with the blue LED photocoagulator EMOLED

Francesca Rossi, Riccardo Cicchi, Francesca Tatini, Stefano Bacci, Domenico Alfieri, Gaetano De Siena, Francesco S. Pavone, Roberto Pini

Author Affiliations +

Proceedings Volume 9542, Medical Laser Applications and Laser-Tissue Interactions VII; 95420F (2015) https://doi.org/10.1117/12.2183670
Event: European Conferences on Biomedical Optics, 2015, Munich, Germany

Abstract

A faster healing process was observed in superficial skin wounds after irradiation with the EMOLED photocoagulator. The instrument consists of a compact handheld photocoagulation device, useful for inducing coagulation in superficial abrasions. In this work we present the results of an in vivo study, in a murine model. Two superficial wounds were produced on the back of 12 mice: one area was left untreated, the other one was treated with EMOLED. Healthy skin was used as a control. The animals were sacrificed 3 hours, 12 hours, 1 day, 6 day after treatment. The treatment effects on back skin was monitored by visual observations, histopathological analysis, immuno-histochemical analysis, and nonlinear microscopic imaging performed at each follow up time, finding no adverse reactions and no thermal damage in both treated areas and surrounding tissues. In addition, a faster healing process, a reduced inflammatory response, a higher collagen content, and a better-recovered skin morphology was evidenced in the treated tissue with respect to the untreated tissue. These morphological features were characterized by means of immuno-histochemical analysis, aimed at imaging fibroblasts and myofibroblasts, and by SHG microscopy, aimed at characterizing collagen organization, demonstrating a fully recovered aspect of dermis as well as a faster neocollagenesis in the treated regions. This study demonstrates that the selective photothermal effect we used for inducing immediate coagulation in superficial wounds is associated to a minimal inflammatory response, which provides reduced recovery times and improved healing process.

Citation Download Citation

Francesca Rossi, Riccardo Cicchi, Francesca Tatini, Stefano Bacci, Domenico Alfieri, Gaetano De Siena, Francesco S. Pavone, and Roberto Pini "Healing process study in murine skin superficial wounds treated with the blue LED photocoagulator EMOLED", Proc. SPIE 9542, Medical Laser Applications and Laser-Tissue Interactions VII, 95420F (15 July 2015); https://doi.org/10.1117/12.2183670

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available