15 July 2015 Healing process study in murine skin superficial wounds treated with the blue LED photocoagulator EMOLED
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Abstract
A faster healing process was observed in superficial skin wounds after irradiation with the EMOLED photocoagulator. The instrument consists of a compact handheld photocoagulation device, useful for inducing coagulation in superficial abrasions. In this work we present the results of an in vivo study, in a murine model. Two superficial wounds were produced on the back of 12 mice: one area was left untreated, the other one was treated with EMOLED. Healthy skin was used as a control. The animals were sacrificed 3 hours, 12 hours, 1 day, 6 day after treatment. The treatment effects on back skin was monitored by visual observations, histopathological analysis, immuno-histochemical analysis, and nonlinear microscopic imaging performed at each follow up time, finding no adverse reactions and no thermal damage in both treated areas and surrounding tissues. In addition, a faster healing process, a reduced inflammatory response, a higher collagen content, and a better-recovered skin morphology was evidenced in the treated tissue with respect to the untreated tissue. These morphological features were characterized by means of immuno-histochemical analysis, aimed at imaging fibroblasts and myofibroblasts, and by SHG microscopy, aimed at characterizing collagen organization, demonstrating a fully recovered aspect of dermis as well as a faster neocollagenesis in the treated regions. This study demonstrates that the selective photothermal effect we used for inducing immediate coagulation in superficial wounds is associated to a minimal inflammatory response, which provides reduced recovery times and improved healing process.
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Francesca Rossi, Francesca Rossi, Riccardo Cicchi, Riccardo Cicchi, Francesca Tatini, Francesca Tatini, Stefano Bacci, Stefano Bacci, Domenico Alfieri, Domenico Alfieri, Gaetano De Siena, Gaetano De Siena, Francesco S. Pavone, Francesco S. Pavone, Roberto Pini, Roberto Pini, } "Healing process study in murine skin superficial wounds treated with the blue LED photocoagulator EMOLED", Proc. SPIE 9542, Medical Laser Applications and Laser-Tissue Interactions VII, 95420F (15 July 2015); doi: 10.1117/12.2183670; https://doi.org/10.1117/12.2183670
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