A SERS-based microfluidic immunoassay using an in-situ synthesized gold substrate

Kequan Fan; Zhuyuan Wang; Lei Wu; Shenfei Zong; Yiping Cui

doi:10.1117/12.2182132

4 May 2015 A SERS-based microfluidic immunoassay using an in-situ synthesized gold substrate

Kequan Fan, Zhuyuan Wang, Lei Wu, Shenfei Zong, Yiping Cui

Proceedings Volume 9543, Third International Symposium on Laser Interaction with Matter; 954319 (2015) https://doi.org/10.1117/12.2182132
Event: Third International Symposium on Laser Interaction with Matter, 2014, Jiangsu, China

Abstract

A sensitive SERS (surface-enhanced Raman scattering)-based immunoassay in microfluidic system has been developed with in-situ synthesis of gold substrate and immune reporter named as 4MBA (4-Mercaptobenzoic acid)-labeled immuno-Ag aggregates. The gold substrate was fabricated simply by introducing the hydrogen tetrachloroaurate (III) trihydrate (HAuCl₄) solution to microchannels using a microfluidic pump. It was found that the obtained deposited gold nanoparticles were uniform in size and shape. Then the sandwich immunoassays were performed using the gold substrates based on SERS signals. In the immunoassay, the gold nanoparticles decorated surface was modified with certain antibodies to recognize the specific kind of antigen, which was flowed through the microfluidic channel afterwards. Then 4MBA-labeled immuno-Ag aggregates were employed as the SERS probes to quantitatively detect the antigen. The experimental results showed a good specificity and limit of detection (LOD) about 1 ng/mL.

Citation Download Citation

Kequan Fan, Zhuyuan Wang, Lei Wu, Shenfei Zong, and Yiping Cui "A SERS-based microfluidic immunoassay using an in-situ synthesized gold substrate", Proc. SPIE 9543, Third International Symposium on Laser Interaction with Matter, 954319 (4 May 2015); https://doi.org/10.1117/12.2182132

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