Translator Disclaimer
5 October 2015 Multi-point optical manipulation and simultaneous imaging of neural circuits through wavefront phase modulation (Presentation Recording)
Author Affiliations +
Abstract
The spatial connectivity of neural circuits and the various activity patterns they exert is what forms the brain function. How these patterns link to a certain perception or a behavior is a key question in neuroscience. Recording the activity of neural circuits while manipulating arbitrary neurons leads to answering this question. That is why acquiring a fast and reliable method of stimulation and imaging a population of neurons at a single cell resolution is of great importance. Owing to the recent advancements in calcium imaging and optogenetics, tens to hundreds of neurons in a living system can be imaged and manipulated optically. We describe the adaptation of a multi-point optical method that can be used to address the specific challenges faced in the in-vivo study of neuronal networks in the cerebral cortex. One specific challenge in the cerebral cortex is that the information flows perpendicular to the surface. Therefore, addressing multiple points in a three dimensional space simultaneously is of great interest. Using a liquid crystal spatial light modulator, the wavefront of the input laser beam is modified to produce multiple focal points at different depths of the sample for true multipoint two-photon excitation.
Conference Presentation
© (2015) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Samira Aghayee, Dan Winkowski, Patrick Kanold, and Wolfgang Losert "Multi-point optical manipulation and simultaneous imaging of neural circuits through wavefront phase modulation (Presentation Recording)", Proc. SPIE 9548, Optical Trapping and Optical Micromanipulation XII, 95481P (5 October 2015); https://doi.org/10.1117/12.2191538
PROCEEDINGS
PRESENTATION ONLY


SHARE
Advertisement
Advertisement
Back to Top