Multiplexed label-free detection of single proteins

Aleksandar Sebesta; Alexandar Weigel; Philipp Kukura

doi:10.1117/12.2188277

26 August 2015 Multiplexed label-free detection of single proteins

Aleksandar Sebesta, Alexandar Weigel, Philipp Kukura

Proceedings Volume 9554, Nanoimaging and Nanospectroscopy III; 95541D (2015) https://doi.org/10.1117/12.2188277
Event: SPIE Nanoscience + Engineering, 2015, San Diego, California, United States

Abstract

We report multiplexed spectral imaging of plasmon resonance shifts in a simple setup, capable of resolving single protein binding events with a high signal to noise ratio of up to 10 for single Fibronectin (450 kDa) proteins. We directly record 2-dimensional, spectrally dispersed images of multiple gold nanorods. The spectra are corrected via an overlapped particle image that correlates arbitrary particle positions to the reference slit position, allowing us to use the full 2D space of the sensor. Together with the simple experimental implementation, our approach paves the way towards routine and efficient label-free detection down to the single molecule level.

Citation Download Citation

Aleksandar Sebesta, Alexandar Weigel, and Philipp Kukura "Multiplexed label-free detection of single proteins", Proc. SPIE 9554, Nanoimaging and Nanospectroscopy III, 95541D (26 August 2015); https://doi.org/10.1117/12.2188277

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