Polarization-dependent single-molecule spectroscopy on photosystem I

S. Skandary; A. Konrad; M. Hussels; A. J. Meixner; M. Brecht

doi:10.1117/12.2207523

26 August 2015 Polarization-dependent single-molecule spectroscopy on photosystem I

S. Skandary, A. Konrad, M. Hussels, A. J. Meixner, M. Brecht

Proceedings Volume 9554, Nanoimaging and Nanospectroscopy III; 95541H (2015) https://doi.org/10.1117/12.2207523
Event: SPIE Nanoscience + Engineering, 2015, San Diego, California, United States

Abstract

Single-molecule spectroscopy (SMS) at low temperature was used to study the spectral properties, heterogeneities and spectral dynamics of the chlorophyll a (Chl a) molecules responsible for the fluorescence emission of photosystem I (PS I). The fluorescence spectra of single PS I complexes are dominated by several red-shifted Chl a molecules categorized into red pools called C708 and C719. By polarization dependent measurements we demonstrate spectrally separate emissions corresponding to C708 and C719 in single PS I monomers and trimers. Moreover, we compared the results of SMS polarization dependent between monomeric and trimeric PS I complexes and give an estimation for the orientation between these red pools. As a consequence, we get new insight into the energy transfer towards and between the red Chl a molecules in PS I complexes.

Citation Download Citation

S. Skandary, A. Konrad, M. Hussels, A. J. Meixner, and M. Brecht "Polarization-dependent single-molecule spectroscopy on photosystem I", Proc. SPIE 9554, Nanoimaging and Nanospectroscopy III, 95541H (26 August 2015); https://doi.org/10.1117/12.2207523

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