21 September 2015 Image reconstruction in serial femtosecond nanocrystallography using x-ray free-electron lasers
Author Affiliations +
Serial femtosecond nanocrystallography (SFX) is a form of x-ray coherent diffraction imaging that utilises a stream of tiny nanocrystals of the biological assembly under study, in contrast to the larger crystals used in conventional x-ray crystallography using conventional x-ray synchrotron x-ray sources. Nanocrystallography utilises the extremely brief and intense x-ray pulses that are obtained from an x-ray free-electron laser (XFEL). A key advantage is that some biological macromolecules, such as membrane proteins for example, do not easily form large crystals, but spontaneously form nanocrystals. There is therefore an opportunity for structure determination for biological molecules that are inaccessible using conventional x-ray crystallography. Nanocrystallography introduces a number of interesting image reconstruction problems. Weak diffraction patterns are recorded from hundreds of thousands of nancocrystals in unknown orientations, and these data have to be assembled and merged into a 3D intensity dataset. The diffracted intensities can also be affected by the surface structure of the crystals that can contain incomplete unit cells. Furthermore, the small crystal size means that there is potentially access to diffraction information between the crystalline Bragg peaks. With this information, phase retrieval is possible without resorting to the collection of additional experimental data as is necessary in conventional protein crystallography. We report recent work on the diffraction characteristics of nanocrystals and the resulting reconstruction algorithms.
© (2015) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Joe P. J. Chen, Joe P. J. Chen, Richard A. Kirian, Richard A. Kirian, Kenneth R. Beyerlein, Kenneth R. Beyerlein, Richard J. Bean, Richard J. Bean, Andrew J. Morgan, Andrew J. Morgan, Oleksandr M. Yefanov, Oleksandr M. Yefanov, Romain D. Arnal, Romain D. Arnal, David H. Wojtas, David H. Wojtas, Phil J. Bones, Phil J. Bones, Henry N. Chapman, Henry N. Chapman, John C. H. Spence, John C. H. Spence, Rick P. Millane, Rick P. Millane, } "Image reconstruction in serial femtosecond nanocrystallography using x-ray free-electron lasers", Proc. SPIE 9600, Image Reconstruction from Incomplete Data VIII, 960002 (21 September 2015); doi: 10.1117/12.2187610; https://doi.org/10.1117/12.2187610


Molecular imaging with x-ray free-electron lasers
Proceedings of SPIE (September 17 2018)
Phase retrieval for crystalline specimens
Proceedings of SPIE (September 05 2017)
Phase retrieval in nanocrystallography
Proceedings of SPIE (October 18 2012)
Phase retrieval from undersampled intensity data
Proceedings of SPIE (October 30 1997)

Back to Top