The method of axial drift compensation of laser differential confocal microscopy based on zero-tracking

Yajie Wang; Han Cui; Yun Wang; Lirong Qiu; Weiqian Zhao

doi:10.1117/12.2193289

5 August 2015 The method of axial drift compensation of laser differential confocal microscopy based on zero-tracking

Yajie Wang, Han Cui, Yun Wang, Lirong Qiu, Weiqian Zhao

Proceedings Volume 9618, 2015 International Conference on Optical Instruments and Technology: Optical Systems and Modern Optoelectronic Instruments; 96180X (2015) https://doi.org/10.1117/12.2193289
Event: International Conference on Optical Instruments and Technology 2015, 2015, Beijing, China

Abstract

Laser differential confocal microscopy (DCM) has advantages of high axial resolution and strong ability of focus identification. However, the imaging mechanism of point scanning needs long measurement time, in the process due to itself mechanical instability and the influence of environment vibration the axial drift of object position is inevitable, which will reduce lateral resolution of the DCM. To ensure the lateral resolution we propose an axial drift compensation method based on zero-tracking in this paper. The method takes advantage of the linear region of differential confocal axial response curve, gets axial drift by detecting the laser intensity; uses grating sensor to monitor the real-time axial drift of lifting stage and realizes closed-loop control; uses capacitive sensor of objective driver to measure its position. After getting the axial drift of object, the lifting stage and objective driver will be driven to compensate position according to the axial drift. This method is realized by using Visual Studio 2010, and the experiment demonstrates that the compensation precision of the proposed method can reach 6 nm. It is not only easy to implement, but also can compensate the axial drift actively and real-timely. Above all, this method improves the system stability of DCM effectively.

Citation Download Citation

Yajie Wang, Han Cui, Yun Wang, Lirong Qiu, and Weiqian Zhao "The method of axial drift compensation of laser differential confocal microscopy based on zero-tracking", Proc. SPIE 9618, 2015 International Conference on Optical Instruments and Technology: Optical Systems and Modern Optoelectronic Instruments, 96180X (5 August 2015); https://doi.org/10.1117/12.2193289

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