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7 August 2015 Detection of Hg2+ in water environment by fluorescence spectroscopic methods
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Abstract
Inorganic mercury (Hg2+) produces toxic effects even at very low concentration. High sensitive fluorescent probes for Hg2+ detection has been researched and synthesized. A fluorescence detection system is built for Hg2+ detection in water environment with fluorescent probes as the detection reagent. Fiber coupled LED with high brightness is developed and used as excitation light source. And the optimized excitation wavelength is about 520 nm. The measurements of fluorescence spectra is obtained by means of optical fiber spectroscopic techniques. Fluorescence detection experiments are carried out for a range of different concentrations of Hg2+ in aqueous solutions. The center wavelength of the fluorescence spectra is about 580 nm which is unchanged in the experiments. Relationship between Hg2+ concentrations and the fluorescence intensity is studied. A positive correlation exists between the intensity of fluorescence spectrum and the concentrations of Hg2+. The fluorescence intensity grows with increasing the concentration of Hg2+ for the same excitation light. When the concentration of Hg2+ is high enough, the fluorescence intensity increases slowly. And a numerical model is built for the concentration calculating. The detection limit is 0.005 μmol/L in the experiments. The Hg2+ detection system reported has many advantages such as small size, rapid response, high-sensitivity, and can be used for on-site testing of the water quality.
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Zhen Li, Jinsong Zhang, Hong Hu, Ruyi Wan, and Youwei Yao "Detection of Hg2+ in water environment by fluorescence spectroscopic methods", Proc. SPIE 9623, 2015 International Conference on Optical Instruments and Technology: Optoelectronic Measurement Technology and Systems, 96230V (7 August 2015); https://doi.org/10.1117/12.2191954
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