High-resolution light-sheet microscopy: a simulation of an optical illumination system for oil immersion

Xiang Lu; Rainer Heintzmann; Ulrich Leischner

doi:10.1117/12.2190823

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23 September 2015 High-resolution light-sheet microscopy: a simulation of an optical illumination system for oil immersion

Xiang Lu, Rainer Heintzmann, Ulrich Leischner

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Proceedings Volume 9626, Optical Systems Design 2015: Optical Design and Engineering VI; 96262I (2015) https://doi.org/10.1117/12.2190823
Event: SPIE Optical Systems Design, 2015, Jena, Germany

Abstract

Light sheet microscopy is a microscopy technique characterized by an illumination from the side, perpendicular to the direction of observation. While this is often used and easy to implement for imaging samples with water-immersion, the application in combination with oil-immersion is less often used and requires a specific optimization. In this paper we present our design of a light-sheet illumination optical system with a ~1μm illumination thickness, a long working distance through the immersion oil, and including a focusing system allowing for moving the focus-spot of the lightsheet laterally through the field of view. This optical design allows for the acquisition of fluorescence images in 3D with isotropic resolution of below 1 micrometer of whole-mount samples with a size of ~1mm diameter. This technique enables high-resolution insights in the 3D structure of biological samples, e.g. for research of insect anatomy or for imaging of biopsies in medical diagnostics.

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Xiang Lu, Rainer Heintzmann, and Ulrich Leischner "High-resolution light-sheet microscopy: a simulation of an optical illumination system for oil immersion", Proc. SPIE 9626, Optical Systems Design 2015: Optical Design and Engineering VI, 96262I (23 September 2015); https://doi.org/10.1117/12.2190823

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