Detection specificity studies of bacteriophage adhesin-coated long-period grating-based biosensor

Marcin Koba; Mateusz Śmietana; Ewa Brzozowska; Sabina Górska; Predrag Mikulic; Andrea Cusano; Wojtek J. Bock

doi:10.1117/12.2194467

28 September 2015 Detection specificity studies of bacteriophage adhesin-coated long-period grating-based biosensor

Marcin Koba; Mateusz Śmietana; Ewa Brzozowska; Sabina Górska; Predrag Mikulic; Andrea Cusano; Wojtek J. Bock

Author Affiliations +

Proceedings Volume 9634, 24th International Conference on Optical Fibre Sensors; 963426 (2015) https://doi.org/10.1117/12.2194467
Event: International Conference on Optical Fibre Sensors (OFS24), 2015, Curitiba, Brazil

Abstract

In this work, we present a label-free detection specificity study of an optical fiber long-period grating (LPG) biosensor working near the dispersion turning point of higher order cladding modes. The LPG sensor functionalized with bacteriophage adhesin is tested with specific and non-specific bacteria dry weight. We show that such biosensor is able to selectively bind, thus recognize different bacteria. We use bacteria dry weights of E. coli B as positive test and E. coli K12 and Salmonella enterica as negative tests. The resonance wavelength shift induced by E. coli B reaches over 90 nm, while for E. coli K12 and Salmonella enterica approximately 40 and 20 nm, respectively.

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Marcin Koba, Marcin Koba, Mateusz Śmietana, Mateusz Śmietana, Ewa Brzozowska, Ewa Brzozowska, Sabina Górska, Sabina Górska, Predrag Mikulic, Predrag Mikulic, Andrea Cusano, Andrea Cusano, Wojtek J. Bock, Wojtek J. Bock, } "Detection specificity studies of bacteriophage adhesin-coated long-period grating-based biosensor ", Proc. SPIE 9634, 24th International Conference on Optical Fibre Sensors, 963426 (28 September 2015); doi: 10.1117/12.2194467; https://doi.org/10.1117/12.2194467

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