Immunobiosensor for fast detection of bacteria in water using plastic optical fiber (POF) bended

Domingos M. C. Rodrigues; Rafaela N. Lopes; Vanessa M. Queiroz; Regina C. S. B. Allil; Marcelo M. Werneck

doi:10.1117/12.2194814

28 September 2015 Immunobiosensor for fast detection of bacteria in water using plastic optical fiber (POF) bended

Domingos M. C. Rodrigues, Rafaela N. Lopes, Vanessa M. Queiroz, Regina C. S. B. Allil, Marcelo M. Werneck

Proceedings Volume 9634, 24th International Conference on Optical Fibre Sensors; 96342M (2015) https://doi.org/10.1117/12.2194814
Event: International Conference on Optical Fibre Sensors (OFS24), 2015, Curitiba, Brazil

Abstract

This paper presents an immunobiosensor of fast response time to detection of bacteria, made by Plastic Optical Fiber. Probes were tested in U-shaped and Meander-shaped to investigate the best sensitivity, accuracy and repeatability. During calibration was used for sucrose solutions refractive index (RI) from 1.33 to 1.39. This is equivalent to IR range of the water and the highest concentration of bacteria, respectively. Immunobiosensor was able to detecting the presence of enteropathogenic Escherichia coli in water from suspensions of different concentrations of 10⁶ and 10⁴colonies forming units per millilitre (CFU/mL) in twenty minutes.

Citation Download Citation

Domingos M. C. Rodrigues, Rafaela N. Lopes, Vanessa M. Queiroz, Regina C. S. B. Allil, and Marcelo M. Werneck "Immunobiosensor for fast detection of bacteria in water using plastic optical fiber (POF) bended", Proc. SPIE 9634, 24th International Conference on Optical Fibre Sensors, 96342M (28 September 2015); https://doi.org/10.1117/12.2194814

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