In this paper, we compare the conventional methods in hydrocarbon seepage anomalies with the signature based detection algorithms. The Crosta technique  is selected as a basement in the experimental comparisons for the conventional approach. The Crosta technique utilizes the characteristic bands of the searched target for principal component transformation in order to determine the components characterizing the target in interest. Desired Target Detection and Classification Algorithm (DTDCA), Spectral Matched Filter (SMF), and Normalized Correlation (NC) are employed for signature based target detection. Signature based target detection algorithms are applied to the whole spectrum benefiting from the information stored in all spectral bands. The selected methods are applied to a multispectral Advanced SpaceBorne Thermal Emission and Radiometer (ASTER) image of the study region, with an atmospheric correction prior to the realization of the algorithms. ASTER provides multispectral bands covering visible, short wave, and thermal infrared region, which serves as a useful tool for the interpretation of the areas with hydrocarbon anomalies. The exploration area is selected as Gemrik Anticline which is located in South East Anatolia, Adıyaman, Bozova Oil Field, where microseeps can be observed with almost no vegetation cover. The spectral signatures collected with Analytical Spectral Devices Inc. (ASD) spectrometer from the reference valley  have been utilized as an input to the signature based detection algorithms. The experiments have indicated that DTDCA and MF outperforms the Crosta technique by locating the microseepage patterns along the mitigation pathways with a better contrast. On the other hand, NC has not been able to map the searched target with a visible distinction. It is concluded that the signature based algorithms can be more effective than the conventional methods for the detection of microseepage induced anomalies.