Ultrafast conformational changes in biomolecules studied by time-resolved circular dichroism

P. Changenet-Barret; F. Hache

doi:10.1117/12.2216989

21 October 2015 Ultrafast conformational changes in biomolecules studied by time-resolved circular dichroism

P. Changenet-Barret, F. Hache

Proceedings Volume 9652, Optics and Photonics for Counterterrorism, Crime Fighting, and Defence XI; and Optical Materials and Biomaterials in Security and Defence Systems Technology XII; 96520Z (2015) https://doi.org/10.1117/12.2216989
Event: SPIE Security + Defence, 2015, Toulouse, France

Abstract

Circular dichroism (CD) is known to be a very sensitive probe of molecular conformation, and it is in particular widely used in biochemistry. Measuring the CD as a function of time is therefore very appealing to access information on the dynamics of conformational changes in molecules or biomolecules. We have implemented such a time-resolved experiments in two complementary configurations: a sub-picosecond pump-probe one and a microsecond detection of CD coupled to a T-jump experiment. We present two experiments based on these techniques: the ultrafast motion of the carbonyl group in the chromophore of the Photoactive Yellow Protein after photoexcitation and the dynamics of thermal denaturation in model peptides.

Citation Download Citation

P. Changenet-Barret and F. Hache "Ultrafast conformational changes in biomolecules studied by time-resolved circular dichroism", Proc. SPIE 9652, Optics and Photonics for Counterterrorism, Crime Fighting, and Defence XI; and Optical Materials and Biomaterials in Security and Defence Systems Technology XII, 96520Z (21 October 2015); https://doi.org/10.1117/12.2216989

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