21 October 2015 Ultrafast conformational changes in biomolecules studied by time-resolved circular dichroism
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Abstract
Circular dichroism (CD) is known to be a very sensitive probe of molecular conformation, and it is in particular widely used in biochemistry. Measuring the CD as a function of time is therefore very appealing to access information on the dynamics of conformational changes in molecules or biomolecules. We have implemented such a time-resolved experiments in two complementary configurations: a sub-picosecond pump-probe one and a microsecond detection of CD coupled to a T-jump experiment. We present two experiments based on these techniques: the ultrafast motion of the carbonyl group in the chromophore of the Photoactive Yellow Protein after photoexcitation and the dynamics of thermal denaturation in model peptides.
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P. Changenet-Barret, P. Changenet-Barret, F. Hache, F. Hache, "Ultrafast conformational changes in biomolecules studied by time-resolved circular dichroism", Proc. SPIE 9652, Optics and Photonics for Counterterrorism, Crime Fighting, and Defence XI; and Optical Materials and Biomaterials in Security and Defence Systems Technology XII, 96520Z (21 October 2015); doi: 10.1117/12.2216989; https://doi.org/10.1117/12.2216989
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