1 July 2015 Absorption and fluorescence spectroscopy on a smartphone
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Proceedings Volume 9655, Fifth Asia-Pacific Optical Sensors Conference; 96551Z (2015) https://doi.org/10.1117/12.2184354
Event: Fifth Asia Pacific Optical Sensors Conference, 2015, Jeju, Korea, Republic of
Abstract
A self-powered smartphone-based field-portable “dual” spectrometer has been developed for both absorption and fluorescence measurements. The smartphone’s existing flash LED has sufficient optical irradiance to undertake absorption measurements within a 3D-printed case containing a low cost nano-imprinted polymer diffraction grating. A UV (λex ~ 370 nm) and VIS (λex ~ 450 nm) LED are wired into the circuit of the flash LED to provide an excitation source for fluorescence measurements. Using a customized app on the smartphone, measurements of absorption and fluorescence spectra are demonstrated using pH-sensitive and Zn2+-responsive probes. Detection over a 300 nm span with 0.42 nm/pixel spectral resolution is demonstrated. Despite the low cost and small size of the portable spectrometer, the results compare well with bench top instruments.
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Md. Arafat Hossain, Md. Arafat Hossain, John Canning, John Canning, Kevin Cook, Kevin Cook, Sandra Ast, Sandra Ast, Peter J. Rutledge, Peter J. Rutledge, Abbas Jamalipour, Abbas Jamalipour, } "Absorption and fluorescence spectroscopy on a smartphone", Proc. SPIE 9655, Fifth Asia-Pacific Optical Sensors Conference, 96551Z (1 July 2015); doi: 10.1117/12.2184354; https://doi.org/10.1117/12.2184354
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