22 December 2015 Low-temperature bonded glass-membrane microfluidic device for in vitro organ-on-a-chip cell culture models
Author Affiliations +
Abstract
The integration of microfluidics with living biological systems has paved the way to the exciting concept of “organson- a-chip”, which aims at the development of advanced in vitro models that replicate the key features of human organs. Glass based devices have long been utilised in the field of microfluidics but the integration of alternative functional elements within multi-layered glass microdevices, such as polymeric membranes, remains a challenge.
To this end, we have extended a previously reported approach for the low-temperature bonding of glass devices that enables the integration of a functional polycarbonate porous membrane. The process was initially developed and optimised on specialty low-temperature bonding equipment (μTAS2001, Bondtech, Japan) and subsequently adapted to more widely accessible hot embosser units (EVG520HE Hot Embosser, EVG, Austria). The key aspect of this method is the use of low temperatures compatible with polymeric membranes. Compared to borosilicate glass bonding (650 °C) and quartz/fused silica bonding (1050 °C) processes, this method maintains the integrity and functionality of the membrane (Tg 150 °C for polycarbonate). Leak tests performed showed no damage or loss of integrity of the membrane for up to 150 hours, indicating sufficient bond strength for long term cell culture. A feasibility study confirmed the growth of dense and functional monolayers of Caco-2 cells within 5 days.
© (2015) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Kyall J. Pocock, Kyall J. Pocock, Xiaofang Gao, Xiaofang Gao, Chenxi Wang, Chenxi Wang, Craig Priest, Craig Priest, Clive A. Prestidge, Clive A. Prestidge, Kazuma Mawatari, Kazuma Mawatari, Takehiko Kitamori, Takehiko Kitamori, Benjamin Thierry, Benjamin Thierry, } "Low-temperature bonded glass-membrane microfluidic device for in vitro organ-on-a-chip cell culture models", Proc. SPIE 9668, Micro+Nano Materials, Devices, and Systems, 96680W (22 December 2015); doi: 10.1117/12.2202461; https://doi.org/10.1117/12.2202461
PROCEEDINGS
9 PAGES


SHARE
Back to Top