Systematic assessment of blood circulation time of functionalized upconversion nanoparticles in the chick embryo

Annemarie Nadort; Liuen Liang; Ekaterina Grebenik; Anna Guller; Yiqing Lu; Yi Qian; Ewa Goldys; Andrei Zvyagin

doi:10.1117/12.2202449

22 December 2015 Systematic assessment of blood circulation time of functionalized upconversion nanoparticles in the chick embryo

Annemarie Nadort, Liuen Liang, Ekaterina Grebenik, Anna Guller, Yiqing Lu, Yi Qian, Ewa Goldys, Andrei Zvyagin

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Proceedings Volume 9668, Micro+Nano Materials, Devices, and Systems; 96683Y (2015) https://doi.org/10.1117/12.2202449
Event: SPIE Micro+Nano Materials, Devices, and Applications, 2015, Sydney, New South Wales, Australia

Abstract

Nanoparticle-based delivery of drugs and contrast agents holds great promise in cancer research, because of the increased delivery efficiency compared to ‘free’ drugs and dyes. A versatile platform to investigate nanotechnology is the chick embryo chorioallantoic membrane tumour model, due to its availability (easy, cheap) and accessibility (interventions, imaging). In our group, we developed this model using several tumour cell lines (e.g. breast cancer, colon cancer). In addition, we have synthesized in-house silica coated photoluminescent upconversion nanoparticles with several functional groups (COOH, NH₂, PEG). In this work we will present the systematic assessment of their in vivo blood circulation times. To this end, we injected chick embryos grown ex ovo with the functionalized UCNPs and obtained a small amount of blood at several time points after injection to create blood smears The UCNP signal from the blood smears was quantified using a modified inverted microscope imaging set-up. The results of this systematic study are valuable to optimize biochemistry protocols and guide nanomedicine advancement in the versatile chick embryo tumour model.

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Annemarie Nadort, Liuen Liang, Ekaterina Grebenik, Anna Guller, Yiqing Lu, Yi Qian, Ewa Goldys, and Andrei Zvyagin "Systematic assessment of blood circulation time of functionalized upconversion nanoparticles in the chick embryo", Proc. SPIE 9668, Micro+Nano Materials, Devices, and Systems, 96683Y (22 December 2015); https://doi.org/10.1117/12.2202449

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KEYWORDS

Blood

Blood circulation

Nanoparticles

Upconversion

In vivo imaging

Content addressable memory

Linear filtering

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