22 December 2015 Acellular organ scaffolds for tumor tissue engineering
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Abstract
Rationale: Tissue engineering (TE) is an emerging alternative approach to create models of human malignant tumors for experimental oncology, personalized medicine and drug discovery studies. Being the bottom-up strategy, TE provides an opportunity to control and explore the role of every component of the model system, including cellular populations, supportive scaffolds and signalling molecules.
Objectives: As an initial step to create a new ex vivo TE model of cancer, we optimized protocols to obtain organ-specific acellular matrices and evaluated their potential as TE scaffolds for culture of normal and tumor cells.
Methods and results: Effective decellularization of animals’ kidneys, ureter, lungs, heart, and liver has been achieved by detergent-based processing. The obtained scaffolds demonstrated biocompatibility and growthsupporting potential in combination with normal (Vero, MDCK) and tumor cell lines (C26, B16). Acellular scaffolds and TE constructs have been characterized and compared with morphological methods.
Conclusions: The proposed methodology allows creation of sustainable 3D tumor TE constructs to explore the role of organ-specific cell-matrix interaction in tumorigenesis.
© (2015) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Anna Guller, Anna Guller, Inna Trusova, Inna Trusova, Elena Petersen, Elena Petersen, Anatoly Shekhter, Anatoly Shekhter, Alexander Kurkov, Alexander Kurkov, Yi Qian, Yi Qian, Andrei Zvyagin, Andrei Zvyagin, } "Acellular organ scaffolds for tumor tissue engineering", Proc. SPIE 9668, Micro+Nano Materials, Devices, and Systems, 96684G (22 December 2015); doi: 10.1117/12.2202473; https://doi.org/10.1117/12.2202473
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