22 December 2015 Liquid marble as microbioreactor for bioengineering applications
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Abstract
This paper reports the use of liquid marbles (LMs) as miniature bioreactors to produce three-dimensional (3D) spheroids including tumor-like spheriods from cancer cells and embryoid bodies (EBs) from stem cells. A liquid marble microbioreactor is prepared by placing a drop of cell suspension onto a polytetrafluoroethylene (PTFE) particle bed. Without the addition of growth factors, suspended EBs from liquid marbles exhibit spontaneous contraction. These results indicate that the liquid marble provides a suitable microenvironment to induce EB formation and spontaneous cardiac differentiation. The EBs were further plated onto gelatin-coated tissue culture dishes. Plated EBs express mature cardiomyocyte marker cardiac troponinT (cTnT), indicating that these EBs have differentiated into functional cardiomyocytes. The cardiomyocytes generated using this liquid marble approach could be useful for transplantation.
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Fatemeh Sarvi, Fatemeh Sarvi, Kanika Jain, Kanika Jain, Layla Alhasan, Layla Alhasan, Tina Arbatan, Tina Arbatan, Wei Shen, Wei Shen, Peggy P. Y. Chan, Peggy P. Y. Chan, } "Liquid marble as microbioreactor for bioengineering applications", Proc. SPIE 9668, Micro+Nano Materials, Devices, and Systems, 966852 (22 December 2015); doi: 10.1117/12.2201110; https://doi.org/10.1117/12.2201110
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