15 October 2015 Maximizing the captured information of integral floating microscopy
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Abstract
To capture the three-dimensional (3D) information of microscopic (micro) object, the light field microscopy (LFM) has been studied. A lens array is inserted into the conventional microscope and 3D information of micro object is captured in single shot. However, since the lateral resolution decreases severely because of lens array, the integral floating microscopy (IFM) is proposed. The IFM is modified version of the LFM which concentrates on the lateral resolution rather than the angular resolution by changing the location of specimen and the lens array. The specimen should be located at the front focal plane and the lens array should be located at the back focal plane of the objective lens in the IFM but it is hard to locate the lens array into the back focal length of the objective lens because the back focal length lies in the barrel of the objective lens in general. In this paper, we propose the modified version of the integral floating microscopy which can place the lens array at the optimum position. The structure of the whole system is changed and the relay lens is added to relay the back focal length outside. By placing the lens array at the optimum position, the captured information could be maximized, and by changing the focal length of the relay lens, the field of view (FOV) mismatch problem can be also mitigated. The relationship between the captured information and the specification of the system is analyzed and proper experiments are presented for the verification.
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Jong-Young Hong, Youngmo Jeong, Jonghyun Kim, Jiwoon Yeom, Byoungho Lee, "Maximizing the captured information of integral floating microscopy", Proc. SPIE 9672, AOPC 2015: Advanced Display Technology; and Micro/Nano Optical Imaging Technologies and Applications, 96720S (15 October 2015); doi: 10.1117/12.2200947; https://doi.org/10.1117/12.2200947
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