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29 February 2016Functional optical coherence tomography for high-resolution mapping of cilia beat frequency in the mouse oviduct in vivo
Since mouse is a superior model for genetic analysis of human disorders, reproductive studies in mice have significant
implications on further understanding of fertility and infertility in humans. Fertilized oocytes are transported through the
reproductive tract by motile cilia lining the lumen of the oviduct as well as by oviduct contractions. While the role of
cilia is well recognized, ciliary dynamics in the oviduct is not well understood, largely owing to the lack of live imaging
approaches. Here, we report in vivo micro-scale mapping of cilia and cilia beat frequency (CBF) in the mouse oviduct
using optical coherence tomography (OCT). This functional imaging method is based on spectral analysis of the OCT
speckle variations produced by the beat of cilia in the oviduct, which does not require exogenous contrast agents. Animal
procedures similar to the ones used for production of transgenic mice are utilized to expose the reproductive organs for
imaging in anesthetized females. In this paper, we first present in vivo structural imaging of the mouse oviduct capturing
the oocyte and the preimplantation embryo and then show the result of depth-resolved high-resolution CBF mapping in
the ampulla of the live mouse. These data indicate that this structural and functional OCT imaging approach can be a
useful tool for a variety of live investigations of mammalian reproduction and infertility.
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Shang Wang, Jason C. Burton, Richard R. Behringer, Irina V. Larina, "Functional optical coherence tomography for high-resolution mapping of cilia beat frequency in the mouse oviduct in vivo," Proc. SPIE 9689, Photonic Therapeutics and Diagnostics XII, 96893R (29 February 2016); https://doi.org/10.1117/12.2210758