Studying the mechanism of neurostimulation by infrared laser light using GCaMP6s and Rhodamine B imaging

David Moreau; Claire Lefort; Sylvia M. Bardet; Rodney P. O'Connor

doi:10.1117/12.2211355

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9 March 2016 Studying the mechanism of neurostimulation by infrared laser light using GCaMP6s and Rhodamine B imaging

David Moreau, Claire Lefort, Sylvia M. Bardet, Rodney P. O'Connor

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Proceedings Volume 9690, Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation; 96901M (2016) https://doi.org/10.1117/12.2211355
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Infrared laser light radiation can be used to depolarize neurons and to stimulate neural activity. The absorption of infrared radiation and heating of biological tissue is thought to be the underlying mechanism of this phenomenon whereby local temperature increases in the plasma membrane of cells either directly influence membrane properties or act via temperature sensitive ion channels. Action potentials are typically measured electrically in neurons with microelectrodes, but they can also be observed using fluorescence microscopy techniques that use synthetic or genetically encoded calcium indicators. In this work, we studied the impact of infrared laser light on neuronal calcium signals to address the mechanism of these thermal effects. Cultured primary mouse hippocampal neurons expressing the genetically encoded calcium indicator GCaMP6s were used in combination with the temperature sensitive fluorophore Rhodamine B to measure calcium signals and temperature changes at the cellular level. Here we present our all-optical strategy for studying the influence of infrared laser light on neuronal activity.

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David Moreau, Claire Lefort, Sylvia M. Bardet, and Rodney P. O'Connor "Studying the mechanism of neurostimulation by infrared laser light using GCaMP6s and Rhodamine B imaging", Proc. SPIE 9690, Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation, 96901M (9 March 2016); https://doi.org/10.1117/12.2211355

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