Gold nanoparticle plasmonics enhanced ultrafast laser-induced optoporation and stimulation of targeted cells (Conference Presentation)

Michel Meunier; Éric Bergeron; Flavie Lavoie-Cardinal; Christos Boutopoulos; Charleen Salesse; Françoise M. Winnik; Paul De Koninck

doi:10.1117/12.2216121

26 April 2016 Gold nanoparticle plasmonics enhanced ultrafast laser-induced optoporation and stimulation of targeted cells (Conference Presentation)

Michel Meunier, Éric Bergeron, Flavie Lavoie-Cardinal, Christos Boutopoulos, Charleen Salesse, Françoise M. Winnik, Paul De Koninck

Author Affiliations +

Proceedings Volume 9690, Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation; 96902J (2016) https://doi.org/10.1117/12.2216121
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Gold nanoparticles (AuNPs) have found numerous applications in nanomedicine in view of their robustness, ease of functionalization and low toxicity. Upon irradiation of AuNPs by a pulsed ultrafast laser, various highly localized phenomena can be obtained including a temperature rise, pressure wave, charge injection and production of nanobubbles close to the cellular membrane [1]. These phenomena can be used to manipulate, optoperforate, transfect and stimulate targeted cells [2-5]. Irradiating at 800 nm in the optically biological transparent window, we demonstrated local optoporation and transfection of cells as well as local stimulation of neurons. Two recent examples will be given: (i) Laser-induced selective optoporation of cells: The technique can be used on various types of cells and a proof of principle will be given on human cancer cells in a co-culture using functionalized AuNPs [6]. (ii) Laser-induced stimulation of neurons and monitoring of the localized Ca2+ signaling: This all optical method uses a standard confocal microscope to trigger a transient increase in free Ca2+ in neurons covered by functionalized AuNPs as well as to measure these local variations optically with the Ca2+ sensor GCaMP6s [7]. The proposed techniques provide a new complement to light-dependent methods in neuroscience. REFERENCES (by our group): (1) Boulais, J. Photochem. Photobiol. C Photochem. Rev. 17, 26 (2013); (2) Baumgart, Biomaterials 33, 2345 (2012); (3) Boulais, NanoLett. 12, 4763 (2012); (4) Boutopoulos, J. Biophotonics (2015); (5) Boutopoulos, Nanoscale 7, 11758 (2015); (6) Bergeron, Biomaterials, submitted (2015); (7) Lavoie-Cardinal, Nature Commun. submitted (2015).

Conference Presentation

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Michel Meunier, Éric Bergeron, Flavie Lavoie-Cardinal, Christos Boutopoulos, Charleen Salesse, Françoise M. Winnik, and Paul De Koninck "Gold nanoparticle plasmonics enhanced ultrafast laser-induced optoporation and stimulation of targeted cells (Conference Presentation)", Proc. SPIE 9690, Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation, 96902J (26 April 2016); https://doi.org/10.1117/12.2216121

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KEYWORDS

Gold

Nanoparticles

Neurons

Plasmonics

Ultrafast phenomena

Biomedical optics

Cancer

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