Imaging human retinal pigment epithelium cells using adaptive optics optical coherence tomography

Zhuolin Liu; Omer P. Kocaoglu; Timothy L. Turner; Donald T. Miller

doi:10.1117/12.2212753

4 March 2016 Imaging human retinal pigment epithelium cells using adaptive optics optical coherence tomography

Zhuolin Liu, Omer P. Kocaoglu, Timothy L. Turner, Donald T. Miller

Author Affiliations +

Proceedings Volume 9693, Ophthalmic Technologies XXVI; 96931E (2016) https://doi.org/10.1117/12.2212753
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Retinal pigment epithelium (RPE) cells are vital to health of the outer retina, but are often compromised in ageing and major ocular diseases that lead to blindness. Early manifestation of RPE disruption occurs at the cellular level, and while biomarkers at this scale hold considerable promise, RPE cells have proven extremely challenging to image in the living human eye. We present a novel method based on optical coherence tomography (OCT) equipped with adaptive optics (AO) that overcomes the associated technical obstacles. The method takes advantage of the 3D resolution of AO-OCT, but more critically sub-cellular segmentation and registration that permit organelle motility to be used as a novel contrast mechanism. With this method, we successfully visualized RPE cells and characterized their 3D reflectance profile in every subject and retinal location (3° and 7° temporal to the fovea) imaged to date. We have quantified RPE packing geometry in terms of cell density, cone-to-RPE ratio, and number of nearest neighbors using Voronoi and power spectra analyses. RPE cell density (cells/mm²) showed no significant difference between 3° (4,892±691) and 7° (4,780±354). In contrast, cone-to- RPE ratio was significantly higher at 3° (3.88±0.52:1) than 7° (2.31± 0.23:1). Voronoi analysis also showed most RPE cells have six nearest neighbors, which was significantly larger than the next two most prevalent associations: five and seven. Averaged across the five subjects, prevalence of cells with six neighbors was 51.4±3.58% at 3°, and 54.58±3.01% at 7°. These results are consistent with histology and in vivo studies using other imaging modalities.

Conference Presentation

Citation Download Citation

Zhuolin Liu, Omer P. Kocaoglu, Timothy L. Turner, and Donald T. Miller "Imaging human retinal pigment epithelium cells using adaptive optics optical coherence tomography", Proc. SPIE 9693, Ophthalmic Technologies XXVI, 96931E (4 March 2016); https://doi.org/10.1117/12.2212753

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

;

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE